Abstract:
Effect of polβ siRNA on Proliferation of Human Ovarian Carcinoma Cell Line and ItsSensitivity to CisplatinLiping HAN1, Juanfang LIU1, Guoqiang ZHAO2, Ziming DONG3Corresponding author: Liping HAN, E-mail: hanliping0825@yahoo.com.cn1Department of Gynecology and Obstetrics, The First Hospital of Zhengzhou University, Zhengzhou 450052, China2Department of Immunology and Microbiology, Medical College of Zhengzhou University, Zhengzhou 450001, China3Department of Pathophysiology, Medical College of Zhengzhou University, Zhengzhou 450001, ChinaAbstract Objective: To investigate the effect of siRNA of polβ on the proliferation of human carcinoma cell (HO-8910) and itssensitivity to cisplatin. Methods: The plasmid expressing siRNA against polβ was constructed and transfected into HO-8910 cells. Thetransfection efficiency was evaluated by fluorescence microscopy. pol β expression was detected by Fluorogenic Quantitative PCR.MTT was used to observe the proliferation of HO-8910 cells and Trypan blue dyeing was used to determine the sensitivity of HO-8910cells to cisplatin. Results: After successful transfection of the constructed siRNA -pol β gene into HO-8910, a large number offluorescent particles were observed in the fluorescence inverted microscope. The expression of pol β in mRNA was significantlyreduced ( P < 0.01 ). The ability of growth and proliferation were reduced. On the other hand, the sensitivity to cisplatin was enhanced( P < 0.05 ). Conclusion: polβ siRNA in ovarian carcinoma cells could induce proliferation of HO-8910 cell line but can enhance itssensitivity to cisplatin.Keywords polβ; siRNA; HO-8910; Cisplatin; Fluorogenic quantitative PCR